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  研究员
 
姓名  
朱蕾蕾
性别  
专家类别  
N/A
职称  
研究员
学历  
博士
电话  
022-24828796
传真  
N/A
电子邮件  
zhu_ll@tib.cas.cn
地址  
天津空港经济区西七道32号
邮编  
300308

简历

1.教育经历

2001.09-2005.06,中国,江南大学,生物工程,学士学位

2005.09-2007.06,中国,江南大学,生物化工,硕士学位

2007.07-2009.06,德国,不来梅雅各布大学(Jacobs University Bremen),生物技术,博士学习

2009.07-2010.09,德国,亚琛工业大学(RWTH Aachen University),生物技术,博士学位

 

2.工作经历

2011.01-2016.07,德国,亚琛工业大学(RWTH Aachen University),研究小组组长 subgroup leader

2016.08-至今,天津工业生物技术研究所,蛋白质定向进化研究组组长


研究方向:
  

发展酶蛋白的定向进化和高通量筛选技术,借助计算机辅助设计等蛋白质分子改造的手段创制高性能的工业酶和蛋白质,结合生物催化和合成生物学手段,推动一碳化合物资源化利用、塑料的生物降解及工业菌株的高效创制等。 

 

代表论著: 

1.论文

 

1.     Ren P., Tan Z., Zhou Y., Tang H., Xu P., Liu H.*, Zhu L*. Biocatalytic CO2 fixation initiates selective oxidative cracking of 1-naphthol under ambient conditions. Green Chemistry,2022,DOI:10.1039/D2GC01226J.

2.     Li T., Tan Z., Tang Z., Liu P., Liu H.*, Zhu L.*, Ma Y. One-pot chemoenzymatic synthesis of glycolic acid from formaldehyde. Green Chemistry,2022,DOI: 10.1039/D2GC00688J.

3.     Cai T.#, Sun H.#, Qiao J.#, Zhu L.#, Zhang F., Zhang J., Tang Z., Wei X., Yang J., Yuan Q., Wang W., Yang X., Chu H., Wang Q., You C., Ma H., Sun Y., Li Y., Li C., Jiang H., Wang Q., Ma Y. Cell-free chemoenzymatic starch synthesis from carbon dioxide. Science,2021,373,1523-1527.(共同第一)

4.     Guo W., Yang J., Huang T., Liu D., Liu Q., Li J., Sun W., Wang X., Zhu L.*, Tian* C. Synergistic effects of multiple enzymes from industrial Aspergillus niger strain O1 on starch saccharification. Biotechnology for Biofuels,2021, 14:225.

5.     Nie Z. #, Liu P.#, Wang Y., Guo X., Tan, Z., Shen J., Tang Z., Sun J., Zheng P. *, Zhu L. * Directed evolution and rational design of mechanosensitive channel MscCG2 for improved glutamate excretion efficiency. Journal of Agricultural and Food Chemistry.2021,69,51,15660-15669.

6.     Yang J., Tu R., Yuan H., Wang Q.*, Zhu L.* Recent advances in droplet microfluidics for enzyme and cell factory engineering. Critical Reviews in Biotechnology,2021,41,7,1023-1045.

7.     Shi L., Liu H., Gao S., Weng Y.*, Zhu L.*. Enhanced extracellular production of IsPETase in Escherichia coli via engineering of the pelB signal peptide. Journal of Agricultural and Food Chemistry.2021,69(7),2245-2252 (ACS Editors’ Choice).

8.     Li T., Tang Z., Wei H., Tan Z., Liu P., Li J., Zheng Y., Lin J., Liu W., Jiang H., Liu H.*, Zhu L.*, Ma Y. Totally atom-economical synthesis of lactic acid from formaldehyde: combined bio-carboligation and chemo-rearrangement without the isolation of intermediate. Green Chemistry, 2020, 22, 6809-6814.

9.     Cheng C., Haider J., Liu P., Yang J., Tan Z., Huang T., Lin J., Jiang M.,* Liu H.*, Zhu L.*. Engineered LPMO significantly boosting cellulase-catalyzed depolymerization of cellulose. Journal of Agricultural and Food Chemistry. 2020, 68, 15257-15266. 

10.   Cheng F., Yang J., Schwaneberg U., Zhu L.*. Rational surface engineering of an arginine deiminase (an antitumor enzyme) for increased PEGylation efficiency. Biotechnology and Bioengineering, 2019, 116(9), 2156-2166.

11.   Cheng F., Yang J., Bocola M., Schwaneberg U., Zhu L.*. Loop engineering reveals the importance of active-site-decorating loops and gating residue in substrate affinity modulation of arginine deiminase (an anti-tumor enzyme).Biochemical and Biophysical Research Communication. 2018, 499(2), 233-238.   

12.   Frauenkron-Machedjou V. J., Fulton A., Zhao J., Weber L., Jaege K. E. r, Schwaneberg U., and Zhu L.*. Exploring the full natural diversity of single amino acid exchange reveals that 40-60% of BSLA positions improve organic solvents resistance. Bioresource and Bioprocessing, 2018, 5:2.    

13.   Markel U.#, Zhu L.#, Frauenkron-Machedjou V. Zhao J., J., Bocola M., Davari M. D., Jaeger K.E., Schwaneberg U. Are Directed evolution approaches efficient in exploring nature’s potential to stabilize a lipase in organic cosolvents? Catalysts, 2017, 7(5), 142.

14.   Charan H., Glebe U., Anand D., Kinzel J., Zhu L., Bocola M., Garakani T. M., Schwaneberg U., Boeker A. Nano-thin walled micro-compartments from transmembrane protein-polymer conjugates. Soft Matter. 2017, 13(15):2866-2875.

15.   Charan H., Kinzel J., Glebe U., Anand D., Garakani T. M., Zhu L., Bocola M., Schwaneberg U., Boeker A. Grafting PNIPAAm from β-barrel shaped transmembrane nanopores. Biomaterials. 2016,107:115-23.

16.   Cheng F.#, Zhu L.#, Schwaneberg U. Directed evolution 2.0: improving and deciphering enzyme properties. Chemical Communication, 2015,51(48):9760-9772.

17.   Cheng F., Kardashliev T., Pitzler C., Shehzad A, Lue H., Bernhagen J, Zhu L.*, Schwaneberg U*. A competitive flow cytometry screening system for directed evolution of therapeutic enzyme. ACS Synthetic Biology, 2015, 4 (7), 768-775.

18.   Zhu L., Cheng F., Piatkowski V. and Schwaneberg U.. Protein engineering of the antitumor enzyme PpADI for improved thermal resistance. ChemBioChem, 2014, 24, 15(2), 276-283.

19.   Hybrid ruthenium ROMP catalysts based on an engineered variant of β-barrel protein FhuA ΔCVF(tev): effect of spacer length. Sauer D. F., Bocola M., Broglia C., Arlt M., Zhu L., Brocker M., Schwaneberg U., Okuda J. Chemistry-An Asian Journal. 2015,10(1):177-82.

20.   Cheng F., Zhu L.*, Lue H., Bernhagen J, Schwaneberg U*. Directed arginine deiminase evolution for efficient inhibition of arginine-auxotrophic melanomas. Applied Microbiology and Biotechnology, 2014, 99(3):1237-1247.

21.   Philippart F., Arlt M., Gotzen S., Tenne S. J., Bocola M., Chen H. H., Zhu L., Schwaneberg U. and Okuda J. A hybrid ring-opening metathesis polymerization catalyst based on engineered ?-barrel protein FhuA. Chemistry an Euopean Journal, 2013, 19(41), 13865-13871.

22.   Liu H., Zhu L., Bocola M., Chen N., Spiess A. C. and Schwaneberg U. Directed laccase evolution for improved ionic liquid resistance. Green Chemistry, 2013, 15, 1348-1355.

23.   Rijn P., Tutus M., Kathrein C., Zhu L., Wessling M., Schwaneberg U., and B?ker A.. Challenges and Advances in the Field of Self-Assembled Membranes, Chemical Society Reviews, 2013, 42(16):6578-6592.

24.   Zhu L., Rajni V., Roccatano D., Ni Y., Sun Z., and Schwaneberg U.. A potential antitumor drug (arginine deiminase) reengineered for efficient operation under physiological conditions. ChemBioChem, 2010, 11(16):2294-301.  

25.   Zhu L., Tee K., Roccatano D., Sonmez B, Ni Y., Sun Z., and Schwaneberg U.. Directed evolution of an antitumor drug (arginine deiminase PpADI) for increased activity at physiological pH. ChemBioChem. 2010, 11, 691-697.  

2. 专著

Zhu L., Arlt M., Liu H., Schwaneberg U. Chapter 3: Channel protein FhuA as a promising biomolecular scaffold for bioconjugates. Bio-Synthetic Hybrid Materials and Bionanoparticles: A Biological Chemical Approach Towards Material Science. Royal Society of Chemistry,ISBN 9781849738224,2015. 

3. 专利

朱蕾蕾、杨建花、谭子瑊.专利号2021102003201

朱蕾蕾、聂志华.专利号:202010062047.6

朱蕾蕾、石利霞、高松枫. 专利号:202010025261.4

朱蕾蕾、李天真、谭子瑊、唐梓静. 专利号:2020109840378

朱蕾蕾、聂志华. 专利号:202010260592.6

朱蕾蕾、成超. 专利号: 201910813543.8


承担科研项目情况:
  

国家重点研发计划子课题

国家自然科学基金青年项目

国家自然科学基金面上项目

天津市自然科学基金面上项目

中国科学院重点部署项目子课题

中国科学院STS项目子课题

中国科学院-诺和诺德项目


获奖及荣誉:
  

2022年获天津市科技系统五一劳动奖章