人才队伍
  您现在的位置:首页 > 中文 > 人才库 > 研究员
  研究员
 
姓名  
游淳
性别  
专家类别  
N/A
职称  
研究员
学历  
博士
电话  
N/A
传真  
N/A
电子邮件  
you_c@tib.cas.cn
地址  
天津空港经济区西七道32号
邮编  
300308

简历

  1、教育经历 

  2003.9-2009.6,复旦大学,微生物遗传学, 博士 

  1999.9-2003.6复旦大学,生物科学,学士     

  2、工作经历 

  2016.3-至今,中国科学院天津工业生物技术研究所,研究员 

  2015.3-2016.3美国, Cell Free Bioinnovations Inc. 首席科学家 

  2012.4-2016.3美国弗吉尼亚理工大学,高级科研助理 

  2010.4-2012.4,美国弗吉尼亚理工大学,博士后

    2009.7-2010.4, 中国科学院天津工业生物技术研究所,助理研究员


研究方向:
  

主要围绕体外生物合成系统组装的适配性、稳定性、有效性等关键科学问题,构建超稳酶元件库,阐明人工设计途径中酶元件的结构与功能关系,创建非天然生物酶,为体外生物合成途径提供特制元件;解析酶元件和人工辅酶识别的分子机制,创制人工辅酶介导的非天然功能模块;揭示多酶复合体物质与能量流传递及分配调控机制,建立多酶复合体的人工设计原则方法;建立非细胞合成途径的工业测试与调控技术,形成人工非细胞合成体系设计与调控标准与原则,为体外生物合成体系设计与定向改造提供理论指导;实现高附加值化学品生成路线的替代,降低高值化学品的生产成本。

 

 

代表论著:

 

1. 论文

2020 

1)      Wang, W., Yang, J., Sun, Y.*, Li, Z.*, and You, C.*. (2020) Artificial ATP-free in vitro Synthetic Enzymatic Biosystems Facilitate Aldolase-mediated C-C Bond Formation for Biomanufacturing, ACS Catalysis 10, 1264-1271. (影响因子12.4)

2)      Meng, D.; Wei, X.; Bai, X.; Zhou, W.; You, C.*, (2020) Artificial in Vitro Synthetic Enzymatic Biosystem for the One-Pot Sustainable Biomanufacturing of Glucosamine from Starch and Inorganic Ammonia. ACS Catalysis, 13809-13819. (影响因子12.4)

3)      Tian, C.; Yang, J.; Li, Y.; Zhang, T.; Li, J.; Ren, C.; Men, Y.; Chen, P.; You, C. *; Sun, Y*.; Ma, Y., (2020) Artificially designed routes for the conversion of starch to value-added mannosyl compounds through coupling in vitro and in vivo metabolic engineering strategies. Metabolic Engineering 2020, 61, 215-224. (影响因子7.8)

4)      Ma, Z.; Li, Q.; Wang, B.; Feng, X.; Xu, H.; Mao, Z.; You, C*.; Sui, X.*, Synthetic Semicrystalline Cellulose Oligomers as Efficient Pickering Emulsion Stabilizer. Carbohydrate Polymers 2020, 117445. Ma, Z.; Li, Q.; Wang, B.; Feng, X.; Xu, H.; Mao, Z.; You, C.; Sui, X., Synthetic Semicrystalline Cellulose Oligomers as Efficient Pickering Emulsion Stabilizer. Carbohydrate Polymers 2020, 117445. (影响因子7.2)

5)      Han, PP., You,C.* (2020) Efficient Multi-enzymes immobilized on porous microspheres for producing inositol from starch. Frontiers in Bioengineering and Biotechnology. 8 (380). (影响因子3.6)

6)      Wei, X.; Han, P.; You, C.*, (2020) Facilitation of cascade biocatalysis by artificial multi-enzyme complexes – A review. Chinese Journal of Chemical Engineering. (影响因子2.0)

7)      Meng, D., Wang, J., and You, C.*. (2020) The properties of the linker in a mini-scaffoldin influence the catalytic efficiency of scaffoldin-mediated enzyme complexes, Enzyme and Microbial Technology. 133, 109460.  (影响因子3.5)

8)      Ma, C.; Liu, M.; You, C.; Zhu, Z. *, Engineering a diaphorase via directed evolution for enzymatic biofuel cell application. Bioresour. Bioprocess. 2020, 7 (1).

 

2019.

9)      Meng, D., Wu, R., Wang, J., Zhu, Z.*, and You, C.* (2019) Acceleration of cellodextrin phosphorolysis for bioelectricity generation from cellulosic biomass by integrating a synthetic two-enzyme complex into an in vitro synthetic enzymatic biosystem, Biotechnol. Biofuels 12, 267. (影响因子5.6)

10)    Li, Y., Liu, S., and You, C.* (2019) Permeabilized Escherichia coli Whole Cells Containing Co-Expressed Two Thermophilic Enzymes Facilitate the Synthesis of scyllo-Inositol from myo-Inositol, Biotechnology Journal 0, 1900191. (影响因子3.6)

11)    Bai, X., Meng, D., Wei, X., Zhou, X., Lu, F., and You, C.*. (2019) Facile synthesis of (?)-vibo-quercitol from maltodextrin via an in vitro synthetic enzymatic biosystem, Biotechnol. Bioeng. 116, 2710-2719. (影响因子4.0)

12)    Wang, W., Meng, D., Li, Q., Li, Z*., and You, C.*. (2019) Characterization of a hyperthermophilic phosphatase from Archaeoglobus fulgidus and its application in in vitro synthetic enzymatic biosystem, Bioresour. Bioprocess. 6:22  (影响因子NA)

13)    Sun, S., Wei, X., and You, C.*. (2019) The construction of an in vitro synthetic enzymatic biosystem that facilitates laminaribiose biosynthesis from maltodextrin and glucose, Biotechnol. J. 14, 1800493. (影响因子3.6)

14)    Song, Y., Liu, M., Xie, L., You, C.*., Sun, J. *, and Zhang, Y.-H. P. J. * (2019) A Recombinant 12-His Tagged Pyrococcus furiosus Soluble [NiFe]-Hydrogenase I Overexpressed in Thermococcus kodakarensis KOD1 Facilitates Hydrogen-Powered in vitro NADH Regeneration, Biotechnol. J.14, 1800301. (影响因子3.6)

15)    Meng, D., Liang, A., Wei, X., and You, C.*. (2019) Enzymatic characterization of a thermostable phosphatase from Thermomicrobium roseum and its application for biosynthesis of fructose from maltodextrin, Appl Microbiol Biot. 103, 6129-6139. (影响因子3.7)

16)    魏欣蕾,游淳*2019)外多酶分子机器的现状和最新进展,生物工程学报. 35(10): 1870?1888

17)    Ma, C., Wu, R., Huang, R., Jiang, W., You, C., Zhu, L., and Zhu, Z. (2019) Directed evolution of a 6-phosphogluconate dehydrogenase for operating an enzymatic fuel cell at lowered anodic pHs, Journal of Electroanalytical Chemistry 851. (影响因子4.3)

18)    Song, H., Ma, C., Liu, P., You, C.., Lin, J., and Zhu, Z. (2019) A hybrid CO2 electroreduction system mediated by enzyme-cofactor conjugates coupled with Cu nanoparticle-catalyzed cofactor regeneration, Journal of CO2 Utilization 34, 568-575. (影响因子5.6)

19)    Zhang, S., Shi, J., Sun, Y., Wu, Y., Zhang, Y., Cai, Z., Chen, Y., You, C., Han, P., and Jiang, Z. (2019) Artificial Thylakoid for the Coordinated Photoenzymatic Reduction of Carbon Dioxide, ACS Catalysis 9, 3913-3925. (影响因子12.4)

 

2018.

20)    Meng, D., Wei, X., Zhang, Y.-H. P. J., Zhu, Z., You, C.*, and Ma, Y. (2018) Stoichiometric conversion of cellulosic biomass by in vitro synthetic enzymatic biosystems for biomanufacturing, ACS Catalysis 8, 9550-9559. (影响因子12.4)

21)    Wei,XL., Xie, L., Zhang, Y.-H. P. J., and You, C.* (2018) Stoichiometric Regeneration of ATP by A NAD(P)/CoA-free and Phosphate-balanced in Vitro Synthetic Enzymatic Biosystem, ChemcatChem, 10, 5597-5601. (影响因子4.6)

22)    Xie, L., Wei, X., Zhou, X., Meng, D., Zhou, R., Zhang, Y.-H. P. J., Xu, S*., and You, C.*. (2018) Conversion of d-glucose to l-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions, Synthetic and Systems Biotechnology. 3, 204-210. (影响因子N.A.)

23)    Shi, T., Han, P., You, C.*, and Zhang, Y.-H. P. J.* (2018) An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design, Synthetic and Systems Biotechnology. 3, 186-195. (影响因子N.A.)

24)    Song, H., Ma, C., Zhou, W., You, C.., Zhang, Y. P. J., and Zhu, Z. (2018) Construction of Enzyme-Cofactor/Mediator Conjugates for Enhanced in Vitro Bioelectricity Generation, Bioconjug Chem 29, 3993-3998. (影响因子4.6)

25)    Zhu, Z., You, C.., Ma, Y., and Zhang, Y.-H. P. J. (2018) In vitro synthetic enzymatic biosystems at the interface of the food-energy-water nexus: A conceptual framework and recent advances, Process Biochem 74, 43-49. (影响因子2.6)

 

2017.

26)    You, C.*, Huang R, Zhu ZG, Zhang Y-HP*. 2017 Protein engineering on nicotinamide-based coenzymes with its applications to synthetic biology. Synthetic and Systems Biotechnology 2017;2: 208-218. (影响因子N.A.)

27)    You, C., Shi T, Li YJ, Han PP, Zhou XG, Zhang Y-HP*. 2017. An in vitro synthetic biology platform for the industrial biomanufacturing of myo-inositol from starch. Biotechnology and Bioengineering 114: 1855-1864. (影响因子4.0)

28)    You, C.*, Zhang Y-HP. 2017. Biomanufacturing by in vitro biosystems containing cascade enzymes. Process Biochemistry. 52: 106-114. (影响因子2.6)

29)     Zhong C, You, C., Wei P, Zhang Y-HP*. 2017. Thermal cycling cascade biocatalysis of myo-inositol synthesis from sucrose. ACS Catalysis. 7: 59925999. (影响因子12.4)

 

2016.

30)    Kim JE, Huang R, Chen H, You, C., Zhang Y-HP. 2016. Facile construction of random gene mutagenesis library for directed evolution without the use of restriction enzyme in Escherichia coli. Biotechnol J. 11:1142-1150. (影响因子3.6)

31)    Zhou W, You, C., Ma H, Ma Y, Zhang YH. 2016. One-Pot Biosynthesis of High-Concentration alpha-Glucose 1-Phosphate from Starch by Sequential Addition of Three Hyperthermophilic Enzymes. J Agric Food Chem. 64:1777-1783. (影响因子2.8)

 

2015.

32)    Rollin JA, Martin del Campo J, Myung S, Sun F, You, C., Bakovic A, Castro R, Chandrayan SK, Wu CH, Adams  MWW, Senger RS, Zhang Y-HP, 2015. High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling. Proceedings of the National Academy of Sciences. 112 (16), 4964-4969. (影响因子9.8)

 

2014.

33)    You, C., Zhang Y-HP*. 2014. Annexation of a high-activity rate-limiting enzyme in a synthetic three-enzyme complex greatly decreases the degree of substrate channeling. ACS Synthetic Biology, 3(6): 380-386  (影响因子5.6)

34)    Zhu ZG, Tam TK, Sun FF, You, C., Zhang Y-HP*. 2014. A high-energy-density sugar biobattery based on a synthetic enzymatic pathway. Nature Communications. 5: 3026, (影响因子13.1)

35)    Myung S, Rollin J, You, C., Sun F, Chandrayan S, Adams MWW, Zhang Y-HP. 2014. In vitro metabolic engineering of hydrogen production at theoretical yield from sucrose. Metabolic Engineering 24(0):70-77. (影响因子7.8)

36)    Peng Q, You, C., Zhang Y-HP*, 2014, One-Pot Enzymatic Conversion of Sucrose to Synthetic Amylose by using Enzyme Cascades. ACS Catalysis. 4:1311-1317. (影响因子12.4)

37)    Gao SH, You, C., Renneckar S, Bao J, Zhang Y-HP*. 2014. New insights into enzymatic hydrolysis of heterogeneous cellulose by using CBM3-containing GFP and CBM17-containing CFP. Biotechnology for Biofuels.  7(1):24  (影响因子5.6)

 

2013.

38)    You, C., Chen HG, Myung S, Sathisuksanoh N, Ma H, Zhang XZ, Li JY, Zhang Y-HP*.2013. Enzymatic transformation of non-food biomass to starch. Proceedings of the National Academy of Sciences of the USA. 110: 7182-7189.  (Highlighted by Science magazine)

39)    You, C., Zhang Y-HP*. 2013. Self-assembly of synthetic metabolons through synthetic scaffoldins: single-step purification, co-immobilization, and substrate channeling. ACS Synthetic Biology 2:102-110, (cover page).

40)    Martin del Campo JS, Rollin JR, Myung S, You, C., Chandrayan S, Adams MWW, Zhang Y-HP*. 2013. Dihydrogen production from xylose and water mediated by synthetic cascade enzymes. Angewandte Chemie International Edition 52:4587-4590,

41)    Myung S, You, C., Zhang Y-HP*. 2013. Recyclable cellulose-containing magnetic nanoparticles: immobilization of cellulose-binding module-tagged proteins and synthetic metabolon featuring substrate channeling. Journal of Materials Chemistry B. 1:4419-4427.

42)    Martin del Campo JS, You, C., Kim J-E, Zhang Y-HP*. 2013. Discovery and characterization of a novel ATP/polyphosphate xylulokinase from a hyperthermophilic bacterium Thermotoga maritima. Journal of Industrial Microbiology and Biotechnology 40:661-669.

 

2012.

43)    You, C., Myung S, Zhang Y-HP*. 2012. Facilitated substrate channeling in a self-assembled trifunctional enzyme complex. Angewandte Chemie International Edition.51: 8787-8790

44)    You, C., Zhang X-Z , Sathitsuksanoh N , Lynd LR, Zhang Y-HP*. 2012. Enhanced microbial cellulose utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complex. Applied and Environmental Microbiology 78(5):1437-1444

45)    You, C., Zhang X-Z, Zhang Y-HP*. 2012. Simple Cloning via direct transformation of PCR product (DNA multimer) to Escherichia coli and Bacillus subtilis. Applied and Environmental Microbiology 78(5):1593-1595

46)    You, C., Zhang Y-HP*. 2012. Easy preparation of a large-size random gene mutagenesis library in Escherichia coli. Analytical Biochemistry 428:7-12

47)    You, C., Zhang X-Z, Zhang Y-HP*. 2012. Mini-scaffoldin enhanced mini-cellulosome hydrolysis performance on low-accessibility cellulose (Avicel) more than on high-accessibility amorphous cellulose. Biochemical Engineering Journal 63:57-65

48)    Xue HP, Zhou JG, You, C., Huang Q, Lu H *. 2012. Amino acid substitutions in the N-terminus, cord and α-helix domains improved the thermostability of a family 11 xylanase XynR8. Journal of Industrial Microbiology and Biotechnology 39:1279-1288

49)    Zhou JG, Bao L, Chang L, Liu Z, You, C., Lu H *. 2012. Beta-xylosidase activity of a GH3 glucosidase/xylosidase from yak rumen metagenome promotes the enzymatic degradation of hemicellulosic xylans. Letters in Applied Microbiology 54:79-87

 

2011.

50)    Zhang Y-HP*, Myung S, You, C., Zhu ZG, Rollin J. 2011. Toward low-cost biomanufacturing through cell-free synthetic biology: bottom-up design. Journal of Materials Chemistry 21: 18877-18886

 

2010.

51)    You, C., Huang Q, Xue HP, Xu Y, Lu H *. 2010. Potential hydrophobic interaction between two cysteines in interior hydrophobic region improves thermostability of a family 11 xylanase from Neocallimastix patriciarum. Biotechnology and Bioengineering 105(5):861-70

52)    You, C.; Yuan, H.; Huang, Q.; Lu, H., Substrate molecule enhances the thermostability of a mutant of a family 11 xylanase from Neocallimastix patriciarum. African Journal of Biotechnology 2010, 9 (9).

 

2. 专著

1)      Wei, X.; Meng, D.; You, C.*, In vitro metabolic engineering: current status and recent progress. Systems and Synthetic Metabolic Engineering2020; p 183-206.

2)      You, C.*, Zhang Y-HP*. Ex Vivo Enzymatic Conversion of Non-food Cellulose Biomass to Starch. In: P. C. K. Lau editor. Quality Living Through Chemurgy and Green Chemistry. Berlin, Heidelberg: Springer Berlin Heidelberg; 2016. p. 129-142.

3)      You, C., Zhang Y-HP*. 2013. Cell-free biosystems for biomanufacturing. Advances in Biochemical Engineering/Biotechnology 131:89-119.

4)      You, C., Zhang Y-HP*. 2014. Simple cloning and DNA assembly in E. coli by prolonged overlap extension PCR. Methods of Molecular Biology 1116

5)      Zhang Y-HP*, You, C., Chen HG, Feng RL. 2012. Surpassing photosynthesis: high-efficiency and scalable CO2 utilization through artificial photosynthesis. ACS Symposium Series 1097: 275-292 (Recent Advances in Post-Combustion CO2 Capture Chemistry), Oxford University Press, UK

6)      Jandt U, You, C., Zhang Y-HP, Zeng A-P*. 2013. Compartmentation and metabolic channeling: practical and modeling aspects for multienzymatic biosynthesis. Advances in Biochemical Engineering/Biotechnology 137:41-65.

7)      Zhong C, You, C., Wei P, Zhang Y-HP*. 2017. Simple cloning by prolonged overlap extension PCR with application to the preparation of large-size random gene mutagenesis library in Escherichia coli. Methods in Molecular Biology.1472: 49-61.

 

3. 专利

11)    吕红;游淳;黄强。一种耐高温耐强碱的木聚糖酶改良基因及其基因工程菌株及其制备方法,申请号:CN200810200060.2

12)   张以恒,游淳。肌醇的制备方法,申请号:201510184621.4 (已授权,和企业合作建成5千吨肌醇产业线)

13)   游淳,魏欣蕾。一种用于ATP再生的底盘系统与应用, 申请号:201711047916.2

14)   游淳,谢雷朋。一种L-乳酸催化反应体系及L-乳酸的制备方法,申请号:201711050815.0

15)   游淳,孙尚尚。一种制备昆布二糖的方法,申请号:201711014505.3

16)   游淳,孙尚尚。一种淀粉转化制备昆布二糖的方法,申请号:201810897084.1

17)   游淳,李元,刘珊。一种鲨肌醇的制备方法,申请号:201810973696.4

18)   游淳,孟冬冬。一种纤维素完全磷酸解产肌醇的方法,申请号:201810972707.7

19)   游淳,李元,刘珊。一种葡萄糖二酸的制备方法,申请号:201811160130.6

20)   游淳,李运杰。酶法催化木糖固定一碳化合物的方法,申请号:201910823381.6


承担科研项目情况:
  
2018年自然科学基金面上项目主持人,中国科学院重点部署项目子课题负责人

获奖及荣誉:
  

2012年 高等学校科学研究优秀成果奖二等奖 

2013上海市科学技术奖二等奖