人才队伍
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  研究员
 
姓名  
张大伟
性别  
专家类别  
N/A
职称  
研究员
学历  
博士
电话  
022-24828749
传真  
N/A
电子邮件  
zhang_dw@tib.cas.cn
地址  
天津空港经济区西七道32号
邮编  
300308

简历

2007年获北京化工大学生物化工专业博士学位。

2007年至2008年,美国威斯康星大学密尔沃基分校,博士后研究

2008年至2012年,美国加州理工学院,博士后研究

2012年至今,中科院天津工业生物技术研究所,研究员,博士生导师


研究方向:
  

1. 微生物代谢与工业菌种创建:主要利用微生物为细胞工厂,采用分子遗传,合成生物技术,生物传感技术,组学技术,高通量筛选等方法,合成维生素,氨基酸,及高附加值化合物。 

2. 蛋白表达系统与蛋白分泌机制:建立蛋白表达平台,包括大肠杆菌,枯草芽孢杆菌,酵母菌表达系统,实现蛋白质胞内表达或分泌表达,为酶制剂和医药蛋白表达提供平台,并开发新型表达系统。基础研究方面主要研究蛋白分泌机制,蛋白分泌新途径,构建新型分泌系统等。 

  

代表论著:

1.      Wu JLiu YZhao SSun JJin Z*, Zhang D*. Application of the Dynamic Regulation to Increase L-Phenylalanine Production in Escherichia coli. J Microbiol Biotechnol. 2019 May 27. doi: 10.4014/jmb.1901.01058  

2.      Jiadi Li#, Xinli Li#, Yuanming Gai, Yumei Sun*, Dawei Zhang*. Evolution of E. coli Phytase for Increased Thermostability Guided by Rational Parameters. Journal of Microbiology and Biotechnology. 2019 DOI:10.4014/jmb.1811.11017

3.      Bai D, Ding D, Li J, Cong L*Zhang D*. Pinpointing the L-phenylalanine binding sites of TyrR using biosensors and computer-aided simulation. Biotechnol Lett. 2019 Jan 24.  

4.      Fu GLiu JLi JZhu BWZhang D*. Systematic screening of optimal signal peptides for secretory production of heterologous proteins in Bacillus subtilis. J Agric Food Chem. 2018 Nov 22. 

5.      Dandan Li#, Gang Fu#, Ran Tu, Zhaoxia Jin* and Dawei Zhang*. High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components. Microb Cell Fact (2019) 18:17

6.      Huan Fang, Dong Li, Jie Kang, Pingtao Jiang, Jibin Sun, Dawei Zhang*. Metabolic engineering of Escherichia coli for de novo biosynthesis of vitamin B12. Nature Communications. (2018) volume 9, Article number: 4917 (Breakthrough)

7.      Cai Y , Xia M, Dong H, Qian Y, Zhang T, Zhu B, Wu J, Zhang D*. Engineering a vitamin B12 high-throughput screening system by riboswitch sensor in Sinorhizobium meliloti.

BMC Biotechnol. 2018 May 11;18(1):27. doi: 10.1186/s12896-018-0441-2.

8.      Yuanming Gai, Jingqi Chen, Shibin Zhang, Beiwei Zhu and Dawei Zhang*. Property Improvement of α-Amylase from Bacillus stearothermophilus by Deletion of Amino Acid Residues Arginine 179 and Glycine 180. Food Technology & Biotechnology. 2018. January 22, doi: 10.17113/ftb.56.01.18.5448.   

9.      Yuanye Chen, Yongfei Liu, Dongqin Ding, Lina Cong*, Dawei Zhang*. Rational design and analysis of an Escherichia coli strain for highefficiency tryptophan production. Journal of Industrial Microbiology & Biotechnology. 2018. February 4 doi.org/10.1007 /s10295 -018-2020-x. 

10.   Liu Y, Xu Y, Ding D, Wen J, Zhu B, Zhang D*. Genetic engineering of Escherichia coli to improve L-phenylalanine production. BMC Biotechnol. 2018 Jan 30;18(1):5. doi: 10.1186/ s12896-018-0418-1. 

11.   Yiran Xu, Yongfei Liu,, Feiran Li, Guoqiang Cao, Ping Zheng, Jibin Sun, Jianping Wen and Dawei Zhang*. Identification of a new gene yecC involved in threonine export in Escherichia coli. FEMS Microbiology Letters. 2017 Sep 15;364(17). doi: 10.1093/femsle/fnx174

12.   Jie Yue, Gang Fu, Dawei Zhang*, Jianping Wen. A new maltose-inducible high-performance heterologous expression system in Bacillus Subtilis. Biotechnol Lett. 2017 Aug;39(8): 1237-1244. doi: 10.1007/s10529-017-2357-7.     

13.   Song, Yafeng; Fu, Gang; Dong, Huina; Li, Jianjun*; Du, Yuguang ; Zhang, Dawei*. High-efficiency secretion of β-mannanase in Bacillus subtilis through protein synthesis and secretion optimization. Journal of Agricultural and Food Chemistry 2017 Mar 29;65(12): 2540-2548, DOI: 10.1021/acs.jafc.6b05528.

14.   Liu, Yongfei; Zhuang, Yinyin; Ding, Dongqin; Xu, Yiran; Sun, Jibin; Zhang, Dawei*. Biosensor-based evolution and elucidation of a biosynthetic pathway in Escherichia coli. ACS Synthetic Biology. 2017 May 19;6(5):837-848. doi: 10.1021/acssynbio.6b00328.

15.   Jingqi Chen; Zhaoxia Jin; Yuanming Gai; Jibin Sun; Dawei Zhang*. A food-grade expression system for D-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase- encoding selection marker. Bioresources and Bioprocessing. 2017;4(1):9. DOI: 10.1186/s40643-017-0139-7.  

16.   Zhao L#, Chen J#, Sun J, Zhang D*, Multimer recognition and secretion by the non-classical secretion pathway in Bacillus subtilis. Scientific Reports. 2017 Mar 9; 7:44023. doi: 10.1038/srep44023.

17.   Huan Fang; Jie Kang; Dawei Zhang*. Microbial production of vitamin B12: A review and future perspectives. Microbial Cell Factories. 2017 Jan 30;16(1):15. doi: 10.1186/ s12934- 017-0631-y. (Highly accessed)

18.   Ding DLiu YXu YZheng PLi HZhang D*, Sun J. Improving the Production of L-Phenylalanine by Identifying Key Enzymes Through Multi-Enzyme Reaction System in Vitro. Scientific Reports. 2016 Aug 25;6:32208. doi: 10.1038/srep32208

19.   Chen JZhu YFu GSong YJin Z, Sun Y*, Zhang D*. High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis. J Ind Microbiol Biotechnol. 2016 Nov;43(11):1577-1591

20.   Song Y?, Nikoloff JM?, Fu G, Chen J, Li Q, Xie N, Zheng P, Sun J, Zhang D*. Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications. PLoS One. 2016 Jul 5;11(7):e0158447. doi: 10.1371/journal. pone.0158447

21.   Jingqi Chen#, Liuqun Zhao#, Gang Fu, Wenjuan Zhou, Yuanxia Sun, Ping Zheng, Jibin Sun and Dawei Zhang*. A novel strategy for protein production using non-classical secretion pathway in Bacillus subtilis. Microbial Cell Factories. 2016 Apr 28;15:69. DOI: 10.1186/s12934-016 -0469-8.

22.   Huina Dong, Sha Li, Huan Fang, Miaomiao Xia, Ping Zheng*, Dawei Zhang*, Jibin Sun. A Newly Isolated and Identified Vitamin B12 Producing Strain—Sinorhizobium meliloti 320. Bioprocess and Biosystems Engineering. 2016 Oct;39(10):1527-37. DOI10.1007/s00449-016-1628-3.

23.   Huan Fang, Huina Dong, Tao Cai, Ping Zheng, Haixing Li, Dawei Zhang* and Jibin Sun. In vitro optimization of enzymes involved in precorrin-2 synthesis using response surface methodology. PloS One. 2016 DOI10.1371/journal.pone.0151149.

24.   Jingqi Chen, Gang Fu, Yuanming Gai, Ping Zheng, Dawei Zhang* and Jianping Wen. Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression. Microbial Cell Factories. 2015, 14:92. (Highly accessed)

25.   Ya’nan Liu, Qinggang Li, Ping Zheng*, Zhidan Zhang, Yongfei Liu, Cunmin Sun, Guoqiang Cao, Wenjuan Zhou, Xiaowei Wang, Dawei Zhang, Tongcun Zhang, Jibin Sun* and Yanhe Ma. Developing a high-throughput screening method for threonine overproduction based on an artificial promoter Microbial Cell Factories. 2015, (2015) 14:121

26.   Yanfei Zhang, Qinglong Meng, Hongwu Ma*, Yongfei Liu, Guoqiang Cao, Xiaoran Zhang, Ping_Zheng, Jibin Sun, Dawei Zhang, Wenxia Jiang, Yanhe Ma. Determination of key enzymes for threonine synthesis through in vitro metabolic pathway analysis, Microbial Cell Factories. 2015. 14:92 .

27.   Jingqi ChenYuanming GaiGang FuWenjuan ZhouDawei Zhang*, Jianping Wen. Enhanced extracellular production of α-amylase in Bacillus subtilis by optimization of regulatory elements and over-expression of PrsA lipoprotein. Biotechnol Lett. 2015 Apr;37(4):899-906. doi: 10.1007/s10529-014-1755-3

28.   Yafeng Song, Jonas M. Nikoloff, Dawei Zhang*. Improving Protein Production on the Level of Regulation both of Expression and Secretion Pathways in Bacillus subtilis. J. Microbiol. Biotechnol. 2015. 25(7): 963~977. 

29.   Huina Dong, Yongfei Liu, Xin Zu, Ning Li, Feiran Li, Dawei Zhang*.An enzymatic assay for high-throughput screening of cytidine-producing microbial strains. PLOS One.  2015 Mar 27;10(3):e0121612. doi: 10.1371/journal.pone.0121612  

30.   Huina Dong, Xin Zu, Ping Zheng, Dawei Zhang*. A rapid enzymatic assay for high-throughput screening of adenosine-producing strains. Microbial Biotechnology. 2015 Mar;8(2):230-8. doi: 10.1111/1751-7915.12189. 

31.   Yongfei Liu, Feiran LiXiaoran ZhangGuoqiang CaoWenxia JiangYuxiu SunPing ZhengDawei Zhang*. A fast and sensitive coupled enzyme assay for the measurement of L-threonine and application to high-throughput screening of threonine-overproducing strains. Enzyme and Microbial Technology. 2014 Dec;67:1-7. doi: 10.1016/j.enzmictec.2014.08.008.

32.   Haifeng Zhang#, Gang Fu#, Dawei Zhang*. Cloning, characterization and production of a novel lysozyme by different expression hosts. J. Microbiol. Biotechnol.2014; 24(10): 1405~1412.

33.   Huina Dong, and Dawei Zhang*. Current development in genetic engineering strategies of Bacillus species, Microb Cell Fact, 2014, 13: 63. (Highly accessed)  

  


承担科研项目情况:
  

1. 国家重点研发计划:高版本模式微生物底盘细胞 

2. 自然基金面上项目:利用SRP抑制子挖掘蛋白转运新途径 

3. 天津市杰出青年科学基金:通用蛋白表达系统构建与机制研究 

4. 中科院科技网络服务STS项目:维生素生物制造

5. 企业重大横向:维生素菌种改造技术


获奖及荣誉:
  

中国产学研合作创新奖 2018

天津市中青年科技创新领军人才 2018

天津市杰出青年科学基金 2017